Was a GT350 ever used in a 007 chase??
No 007 films did not use any Shelby GT350.
But these movies did use real vintage Shelbys;
1968 Shelby GT500, Thomas Crown AffairIn this remake of the Steve McQueen classic, billionaire Thomas Crown, played by Pierce Brosnan, drives a one-off, off-road prepped 1968 Shelby Mustang GT500 convertible complete with roll bar, raised suspension, and knobby tires. It isn’t in the movie long, but it’s unforgettable. Today a faithful replica owned by reality TV star Richard Rawlings is often seen on his hit show Fast n’ Loud.
1966 Shelby GT350H, Grand PrixFilmed throughout Europe with real F1 cars on real race tracks, including Monaco and the high banks of Monza, Grand Prix remains the prototypical racing movie. American F1 driver Pete Aron, played by car enthusiast and real-life racer James Garner, drives a black and gold 1966 Shelby GT350H Mustang hard when not on the track. A similar car—a clone—appears in the Tom Cruise remake of The War of the Words from 2005.